Coding

Part:BBa_K5048052:Design

Designed by: Yiheng Liao   Group: iGEM24_WHU-China   (2024-09-22)


lasR-dt-plas-ccdB-dt


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 322
    Illegal AgeI site found at 519
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1194


Design Notes

We design this part in order to reproduce the result of the literature, except for the double terminators. we discussed with CDG China and they recommended this part because it had high efficiency.



Source

complete synthesis

References